| Cat. No. | Volume | |
| N2 Supplement | F005-004 | 5 ml |
Applications
N2 Supplement is an optimised serum free supplement used in combination with NeuroMix for:
- Differentiation of ES cells into neuron lineage (neuron and astrocytes)
- Differentiation of neural stem cells into astrocytes and neurons
- Optimal for serum free growth for neuroblastomas
- Use in combination with NeuroMix
Shelf Life and Storage
N2 Supplement is stable for 1 year when stored at ?-15°C.
Instructions for use
N2 Supplement is a 100 fold concentrate. Dilute N2 Supplement (100x) in the base medium 1:100. The final concentration of N2 Supplement corresponds to 1x. For preparation of 100 ml medium add 1 ml N2 Supplement (100x) into 99 ml of the appropriate base medium.
Cell culture vessels must be coated with poly-D-lysine (0.05 mg/ml). Fibronectin must be added at a final concentration of 5-10 µg/ml directly to the medium.
For serum free growth of neuroblastomas add N2 Supplement (100x) into Neuronal Base Medium (supplemented with 0.5 mM L-glutamine and 25 µM glutamate) to a final concentration of 1x.
For in vitro laboratory use or further manufacturing only. Not for human use.