Cat. No. Volume
N2 Supplement F005-004 5 ml

Applications

N2 Supplement is an optimised serum free supplement used in combination with NeuroMix for:

  • Differentiation of ES cells into neuron lineage (neuron and astrocytes)
  • Differentiation of neural stem cells into astrocytes and neurons
  • Optimal for serum free growth for neuroblastomas
  • Use in combination with NeuroMix

Shelf Life and Storage

N2 Supplement is stable for 1 year when stored at ?-15°C.

Instructions for use

N2 Supplement is a 100 fold concentrate. Dilute N2 Supplement (100x) in the base medium 1:100. The final concentration of N2 Supplement corresponds to 1x. For preparation of 100 ml medium add 1 ml N2 Supplement (100x) into 99 ml of the appropriate base medium.

Cell culture vessels must be coated with poly-D-lysine (0.05 mg/ml). Fibronectin must be added at a final concentration of 5-10 µg/ml directly to the medium.

For serum free growth of neuroblastomas add N2 Supplement (100x) into Neuronal Base Medium (supplemented with 0.5 mM L-glutamine and 25 µM glutamate) to a final concentration of 1x.

For in vitro laboratory use or further manufacturing only. Not for human use.