Cat. No.	Volume
Collagenase 2	K21-240	1 g
Collagenase 2
dialysed, sterile	K02-239	20 ml

Collagenase cleaves peptide bonds in native, triple-helical collagen. The substrate, collagen, is the major fibrous component of animal extracellular connective tissue: skin, tendon, blood vessels, bone, etc. True collagenase may cleave simultaneously across all three chains or attack a single strand.

Activator: Ca ²⁺

Inhibitor: EDTA/EGTA (no inactivation by serum)

pH-optimum: 7.0 - 8.0

Activity (Wünsch): ≥ 0.1 Units*/mg

*(One unit catalyses the hydrolysis of 1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucyl-glycyl-L-prolyl-D-arginine per minute at +25°C, pH 7.1)

Reconstitution: In water (100 mg/ml)

Concentration: 2 Units*/ml

Powder: The lyophilisat is stable for up to 2 years at +2 °C to +8 °C

Solution: The reconstituted solution is stable for 2 years at ≤-15 °C

Use Collagenase 2 in the same manner that trypsin EDTA solutions are used to detach adherent cells from surfaces. Always handle cells with standard aseptic techniques if the cells are subcultured or propagated further.

1. Wash the cells with a small volume of sterile PBS. Remove wash liquid.
2. Add appropriate amount of Collagenase 2.
3. Return the culture to +37°C incubator and allow cells to detach for about 5-20 minutes.
4. Count cells and passage as usual.

1. Incubation of tissue with an appropriate amount of Collagenase 2 in culture medium.
2. Return to the CO₂-incubator for 1-48 hours (depending on the type of tissue).
3. Remove the dissociated cells from the tissue.
4. Cultivate the cells as usual.

For in vitro laboratory use or further manufacturing only. Not for human use.