| Cat. No. | Volume | |
| Neuronal Base Medium | ||
| without L-Glutamine | U15-023 | 500 ml |
Neuronal Base Medium is optimised for:
- For cultivation and long term viability of foetal neurons
- For serum free cultivation of neuroblastomas
- For differentiation of neuronal stem cells into astrocytes and neurons
- For expansion of neuronal stem stells (avoids differentiation)
For applications where serum is necessary, start with 5 % serum in Neuronal Base Medium. Cell culture vessels must be coated with poly-D-lysine (0.05 mg/ml). If using N2 Supplement add fibronectin at a final concentration of 5-10 µg/ml directly to the medium. For applications where serum is necessary, start with 5 % serum in Neuronal Base Medium. Cell culture vessels must be coated with poly-D-lysine (0.05 mg/ml). If using N2 Supplement add fibronectin at a final concentration of 5-10 µg/ml directly to the medium.
Composition
Neuronal Base Medium is an optimised base medium with neuronal specific trace elements but without L-glutamine (must be supplemented).
Shelf Life and Storage
Neuronal Base Medium has a shelf life of 18 months when stored at +2°C to +8°C.
For cultivation and long term viability of foetal neurons
- Neuronal Base Medium must be supplemented with 0.5 mM L-glutamine (for primary cells) or 2.0 mM L-glutamine (for other neuronal cell types).
- For serum-free cultivation Neuronal Base Medium must be supplemented with NeuroMix or with N2 Supplement. For initial plating of embryonic primary hippocampal neurons 25 µM glutamate must be added for the first 4 days. After initial plating glutamate is not necessary.
For serum free cultivation of neuroblastomas
Add N2 Supplement into Neuronal Base Medium (supplemented with 25 µM glutamate) to get a final concentration of 1x.
For differentiation of neuronal stem cells into astrocytes and neurons
Add NeuroMix (and G5 Supplement) into Neuronal Base Medium (add 2 mM L-glutamine) to get a final concentration of 1x.
For expansion of neuronal stem stells (avoiding differentiation)
Add Neuronal Stem Cell Supplement (and G5 Supplement) into Neuronal Base Medium (add 2 mM L-glutamine) to get a final concentration of 1x.
Products for long-term viability of hippocampal and other neurons of the CNS
| Product, Cat No. | Volume | Application |
| Neuronal Base Medium, U15-023 | 500 ml | Base medium for long-term growth and viability growth of rat hippocampal neurons |
| For survival of neurons from embryonic rat striatum, substantia nigra, septum and cortex, and neonatal rat cerebellum | ||
| Must be used in combination with NeuroMix | ||
| NeuroMix (50x), F01-002 | 10 ml | As supplement for long-term growth and viability growth of rat hippocampal neurons |
| As supplement for cultivation of rat postnatal and adult cortical neurons | ||
| In combination with Neuronal Base Medium AD | ||
| Must be diluted 1:50 | ||
| N2-Supplement (100x), F005-004 | 5 ml | As supplement for tumor cell lines of neuronal origin |
| In combination with Neuronal Base Medium AD | ||
| Must be diluted 1:100 | ||
| G5 Supplement (100x), F001-003 | 1 ml | In combination with Neuronal Base Medium AD |
| As additional supplement with NeuroMix (dilution 1:50) in combination with Neuronal Base Medium AD | ||
| Must be diluted 1:100 |
For in vitro laboratory use or further manufacturing only. Not for human use.