Cat. No.	Volume
MesenchymStem Medium
with L-Glutamine	U15-828	500 ml

MesenchymStem Medium is a ready to use medium for cultivation and expansion of mesenchymal stem cells. No supplementation of serum and L-glutamine is required.

MesenchymStem Medium consists of a modified DMEM medium containing specific trace elements, FBS and L-glutamine.

MesenchymStem Medium is stable for 2 years when stored at <-15°C. Once thawed, MesenchymStem Medium is stable for 4 weeks when stored at +2°C to +8°C.

MesenchymStem Medium should be thawed in a +37°C water bath and mixed by swirling prior to use.  Alternatively the medium can also be thawed overnight at +2°C to +8°C.

If required antibiotics can be added. After thawing the complete medium can be dispensed into appropriate vessels and mesenchymal stem cells can be transferred. Incubate cells in an incubator with an optimal CO₂-concentration of 8.5 % at 37°C.

For differentiation of mesenchymal stem cells into bone-, cartilage-, muscle- and fat cells cytokines and growth factors must be added (not included).

Product	Cat. No.	Application
FBS pre-tested on ES Cells	A15-208	As supplement for growth of mesenchymal stem cells
MesenchymStem Medium	U15-828	Complete medium for expansion of mesenchymal stem cells
		For differentiation in chondrocytes, osteoblasts and adipocytes growth factors must be added
DMEM, low Glucose,without L-glutamine	E15-005	Base medium for growth of mesenchymal stem cells (glutamine must be added)
Accutase	L11-007	For gentle dissociation of mesenchymal stem cells
Trypsin EDTA	L11-004	For dissociation of mesenchymal stem cells

For in vitro laboratory use or further manufacturing only. Not for human use.