| Cat. No. | Volume | |
| MesenchymStem Medium | ||
| with L-Glutamine | U15-828 | 500 ml |
MesenchymStem Medium is a ready to use medium for cultivation and expansion of mesenchymal stem cells. No supplementation of serum and L-glutamine is required.
Composition
MesenchymStem Medium consists of a modified DMEM medium containing specific trace elements, FBS and L-glutamine.
Shelf Life and Storage
MesenchymStem Medium is stable for 2 years when stored at <-15°C. Once thawed, MesenchymStem Medium is stable for 4 weeks when stored at +2°C to +8°C.
Thawing
MesenchymStem Medium should be thawed in a +37°C water bath and mixed by swirling prior to use. Alternatively the medium can also be thawed overnight at +2°C to +8°C.
Incubation
If required antibiotics can be added. After thawing the complete medium can be dispensed into appropriate vessels and mesenchymal stem cells can be transferred. Incubate cells in an incubator with an optimal CO2-concentration of 8.5 % at 37°C.
For differentiation of mesenchymal stem cells into bone-, cartilage-, muscle- and fat cells cytokines and growth factors must be added (not included).
Products for mesenchymal stem cell cultivation
| Product | Cat. No. | Application |
|---|---|---|
| FBS pre-tested on ES Cells | A15-208 | As supplement for growth of mesenchymal stem cells |
| MesenchymStem Medium | U15-828 | Complete medium for expansion of mesenchymal stem cells |
| For differentiation in chondrocytes, osteoblasts and adipocytes growth factors must be added | ||
| DMEM, low Glucose,without L-glutamine | E15-005 | Base medium for growth of mesenchymal stem cells (glutamine must be added) |
| Accutase | L11-007 | For gentle dissociation of mesenchymal stem cells |
| Trypsin EDTA | L11-004 | For dissociation of mesenchymal stem cells |
For in vitro laboratory use or further manufacturing only. Not for human use.