Stem Cell Culture

PAA offers a large range of different basal media, growth supplements and reagents for the cultivation of embryonic, hematopoietic, neuronal and mesenchymal stem cells. The extensive applications in the research area of stem cell cultivation include among others:

• Growth and maintenance of mesenchymal stem cells

• Growth and in vitro differentiation of hematopoietic stem cells and their progenitors
• Cytokine-directed formation of hematopoietic progenitors in semi-solid media
• Long-term growth of hematopoietic progenitors (myeloid cells)
• Formation of embryoid bodies as precursors for hematopoietic cells
• Growth and maintenance of undifferentiated ES cells for in vitro differentiation to hematopoietic cells and progenitors

• Growth and maintenance of undifferentiated ES cells for in vitro differentiation to hematopoietic or neuronal cells
• Formation of embryoid bodies
• Growth and maintenance of human ES cells for possible re-implantation experiments
• Cytokine-directed formation of hematopoietic colonies in semi-solid media

• Growth, differentiation and maintenance of neuronal stem cells
• Cytokine-directed formation of cells of neuron lineages

Products	Embryonic Stem Cells	Neuronal Stem Cells	Mesenchymal Stem Cells	Hematopoietic Stem Cells
FBS ES cell tested	x		x	x
Horse Serum				x
Human Serum Type AB, otc	x
MethoStem Medium	x	x		x
CollagenStem Kit				x
MesenchymStem Medium			x
Neuronal Base Medium	x	x
IMDM	x			x
MEM Alpha Mod.				x
DMEM, low Glucose			x
DMEM, high Glucose, with sodium pyruvate	x
DMEM/Ham's F-12	x	x
HematoStem	x			x
B27 NeuroMix	x	x
G5 Supplement	x	x
N2 Supplement		x
Neuronal Stem Cell Supplement		x