Cat. No.	Volume
SILAC DMEM
w/o L-Arginine, w/o L-Lysine, w/o L-Glutamine	E15-086	500 ml

Stable Isotope Labelling with Amino acids in Cell culture (SILAC) is an in vivo labelling method for relative quantization of proteins. A mass spectrometry-based technique was developed to detect differences in protein abundance between two (or more) samples. Therefore two populations of cells are cultivated in cell culture with either normal amino acids or the corresponding heavy isotopes. Proteins from both cell populations can be combined and analyzed by mass spectrometry. 

• Quantitative analysis of relative changes in protein expression
• Quantitative analysis of proteins for which no antibodies are available
• Protein expression of normal cells compared to disease cells
• Identification and quantification of a high number of proteins in a single experiment
• Analysis of signalling pathways

• Non radioactive stable isotope labelling
• 100% label incorporation into proteins of living cells
• No intra-experimental variability caused by different sample preparations
• Media deficient in both L-lysine and L-arginine

CO2-Concentration, optimum	8.5 %
pH	6.8 – 7.5
Osmolality	280 – 350 mOsmol/kg
Endotoxin	< 1 EU/ml
Cell Culture	tested
Sterility	tested
Shelf Life	18 months
Storage	+2°C to +8°C