Cat. No.	Volume
Puromycin	P11-019	100 mg
	P15-019	500 mg

Puromycin is an aminonucleoside antibiotic isolated from Streptomyces alboniger. It specifically inhibits the peptidyl transfer on ribosomes of prokaryotic and eukaryotic cells. Puromycin inhibits the growth of gram positive bacteria as well as various animal and insect cells and is used for the selection of mammalian cells which have been transformed by vectors expressing puromycin-N-acetyl-transferase.

Puromycin resistance to mammalian cells is conferred by the Streptocmyces alboniger gene pac which is coding for the puromycin N-acetyl-transferase (PAC). The pac gene is located in a region of the pur cluster linked to other genes determining the puromycin bio­synthetic pathway.

Puromycin is usually used in experiments conducted with mammalian cells. It can be useful as an alternative to the neomycin system for transfection experiments.
In some particular conditions Puromycin can also be used for the selection of E.coli transformants but it is poorly active on E.coli.

Formula: C₂₂H₂₉N₇O₅ x 2HCl

Molecular Weight: 544.2 g/mol

The recommended working concentration of Puromycin is 1 to 10 µg/ml.