Cat. No. Volume
Puromycin P11-019 100 mg
  P15-019 500 mg

General

Puromycin is an aminonucleoside antibiotic used for selection of mammalian cell lines expressing the puromycin resistance gene. The antibiotic is active against gram positive bacteria and various animal and insect cells by inhibiting the peptidyl transfer on prokaryotic and eukaryotic ribosomes. Transfected cells become resistant against puromycin by expression of the pac gene encoding a puromycin N-acetyl transferase.

Applications

  • Selection antibiotic for transfection
  • Spectrum: gram positive bacteria, various animal and insect cells
  • Selection of cell types harbouring plasmids carrying puromycin resistance genes
  • Can be used as an alternative to the neomycin system for transfection experiments

Features

  • Aminoglycoside antibiotic
  • Inhibition of peptidyl transfer
  • High activity
  • High purity

Chemical Properties

Formula: C22H29N7O5 x 2HCl

Molecular Weight: 544.2 g/mol

Working Concentration

Recommended final concentration: 1 – 10 µg/ml  

Product Specifications

Purity > 98.0 %
Shelf Life 3 years
Storage +2°C to +8°C