Nanofectin Kit
| Cat. No. | Volume | |
| Nanofectin Kit | Q050-005 | |
| Nanofectin | 0.5 ml | |
| Diluent | 50 ml | |
| Nanofectin Kit | Q051-005 | |
| Nanofectin | 1.0 ml | |
| Diluent | 50 ml |
General
Different transfection methods are used for the transfer of nucleic acids into mammalian cells. The transfection efficiency depends on the size of the particle to be taken up by the cells and on the DNA stability.
Nanofectin is a transfection reagent for optimal uptake of nucleic acids into the cell which can be used in the presence of serum. DNA stability is ensured as it is bound into the porous nanoparticles where it is protected against endonucleolytic degradation.
PAA provides Nanofectin as a kit system in combination with an optimized diluent.
Applications
- Transfection reagent for mammalian cells
- Transfection of DNA
- Broad cell range reagent
- Ideal for adherent and suspension cells
- Successful for transfection of difficult cells
- For transient and stable expression
Features
- Provided as kit system
- Consists of a positively charged polymer and porous nanobeads
- Protection of bound DNA against endonucleolytic degradation
- Efficient binding of DNA
- Uniform size for optimal uptake into the cell
- Easy to use
- High transfection rate
- Transfection in presence of serum
- Tested with several cell lines
- Non toxic
Product Specifications
| Solvent Nanofectin | Water |
| Diluent | NaCl |
| Endotoxin | < 1 EU/ml |
| Cell Culture | tested |
| Sterility | tested |
| Shelf Life | 1 year |
| Storage | +2°C to +8°C |