| Cat. No. | Volume | |
| Nanofectin Kit | Q050-005 | |
| Nanofectin | 500 µl | |
| Diluent | 50 ml | |
| Nanofectin Kit | Q051-005 | |
| Nanofectin | 1000 µl | |
| Diluent | 50 ml |
Requirements for Transfection Reagents
Transfection reagents differ considerably in transfection efficiency. Most reagents transfer DNA inefficiently as cells only take up particles of a specific size. In addition, most reagents cannot sufficiently protect the DNA-complex against endonuclease degradation. New carrier molecules developed by PAA allow the DNA-complexes to be taken up more efficiently, thus increasing the transfection rate.
The Development
Nanofectin consists of two components, a positively charged polymer which is coiled into a porous nanoparticle. The specific particle size and structure of Nanofectin allows DNA to be taken up by cells more efficiently. By the principle of surface area enlargement, the DNA binding capacity is increased.
Uptake of the DNA-nanoparticle Complex
Modern nanotechnology enables the production of nanoparticles of uniform size for an optimized uptake of the DNA-nanoparticle complex.
Nanoparticles protect DNA
After transfection and release of the DNA-nanoparticles into the cell cytoplasm, the structure of Nanofectin protects the bound DNA against endonucleolytic degradation. This allows for successful transfection using lower quantities of DNA.
Nanofectin for stable and transient Expression
DNA bound to Nanofectin can pass the nuclear membrane. Within the nucleus, stable integration into the chromosome can occur. Transient expression in the cytoplasm is also extended with the use of Nanofectin.
Easy Transfection
Nanofectin is extremely easy to use. The DNA has to be diluted with PAA’s Diluent and incubated with Nanofectin for 30 minutes before the mixture is added to the appropriate cells. The expression is measured after 24-48 hours. Nanofectin can be used in the presence of serum so there is no need to rinse cells before incubation with the Nanofectin-DNA complexes.
Features and Benefits
- Easy to use
- Transfection in the presence or absence of serum
- Effective for stable and transient transfection
- High transfection rates
- Tested on primary, adherent- and suspension cells
- Non-toxic
- Stable for 12 months
- Ready to use kit containing PAA’s Diluent