| Cat. No. | Volume | |
| MethoStem Medium | U11-827 | 100 ml |
General
For cultivation of hematopoietic stem cells a variety of different semi-solid culture systems have been developed. Methylcellulose is widely used as a gelling agent which increases the viscosity of the medium without converting it to a solid. Methylcellulose-based media are used in addition to optimal levels of growth factors for the cultivation of hematopoietic and embryonic stem cells and their progenitors. In addition methylcellulose increases the viscosity of the medium to allow colony formation of different progenitor cells for simplified separation and identification. For further differentiation of the stem cells cytokines must be added.
Methylcellulose-based cultures cannot be fixed for cytochemical or immunocytochemical staining. Therefore collagen-based media are recommended.
Composition
MethoStem Medium consists of a modified IMDM medium supplemented with methylcellulose, BSA, 2-mercaptoethanol and L-glutamine. FBS (10–30%) and cytokines have to be added before usage.
Features and Benefits
- Growth and differentiation of hematopoietic stem cells
- Differentiation and characterization of stem cell progenitors
- For further cultivation and simplified separation of single cells by colony selection
- Growth of embryonic stem cells