Cat. No.	Volume
MesenchymStem Medium	U15-828	500 ml

Mesenchymal stem cells, part of the hematopoietic stem cell network, have become a more and more important part of research into human therapeutics. Mesenchymal stem cells, derived from bone marrow, are capable of self-renewal and differentiation into bone-, cartilage-, muscle- and fat cells. Differentiation into distinct cell types can be achieved by directed stimu­lation using cytokines. In vivo tissue repair using differentiated cells is possible.

Mesenchymal stem cells can be prepared after sepa­ration of mononuclear cells by density gradient centri­fugation. Proliferation of mesenchymal stem cells can be increased by using media enriched with specific growth factors from FBS. MesenchymStem Medium developed by PAA provides the optimal concentration of growth factors needed for expansion of mesenchy­mal stem cells and their progenitors.

For further differentiation into adipocytes, chondro­cytes or osteocytes different additives, such as dexa­methasone, isobutyl-methylxanthine, ascorbic acid phosphate and indomethacin must be added to MesenchymStem Medium.

MesenchymStem Medium is a modified DMEM medi­um with specific trace elements, FBS and L-glutamine. For differentiation of mesenchymal cells into bone-, cartilage-, muscle- and fat cells cytokines must be added.

• Ready to use medium
• Optimized formulation for expansion of mesenchy¬mal cells
• Simplified characterization and quantification of mesenchymal progenitors
• Optimal medium for further differentiation experi¬ments