Cat. No.	Volume
InsectExpress Sf9-S2
with L-Glutamine	E15-875	500 ml

The baculovirus expression system is the most effective expression system of eukaryotic genes. Protein titres of more than 500 mg/l are achievable. Serum free media are free from batch to batch variation of serum. Protein free media additionally show the advantage of easy purification of the expressed protein. PAA offers with its Insect Express Sf9-S2 a protein free medium, which is optimized for the cultivation of Spodoptera frugiperda and Drosophila S2 cells.

• Optimized for cultivation of Spodoptera frugiperda and Schneider´s S2 drosophila cells
• Developed for the baculovirus expression system with BEVS
• Suitable for adherent as well as suspension cells in bioreactors

• Protein free complete medium
• Fully defined
• Growth densities higher than 10⁷ cells/ml
• High glucose content
• With Pluronic F68 for minimizing shear forces
• Without hypoxanthine and thymidine
• Hydrolysate source from yeast
• Without HEPES
• Without phenol red

• Proteins will be modified posttranslationally
• Cytoplasmatic as well as cell surface proteins can be expressed
• High specific productivity
• No adaptation from cell cultures containing serum necessary
• No feeding necessary for 10 days
• Increased yield
• Suitable as freezing medium

For application in larger units (fermenters) the Insect Express Sf9-S2 can also be offered in customized sizes. Adapters, connectors and volumes can be individually configured.

CO2-Concentration, optimum	0.0 %
pH	6.0 – 7.0
Osmolality	350 – 400 mOsmol/kg
Endotoxin	< 20 EU/ml
Cell Culture	tested
Sterility	tested
Shelf Life	12 months
Storage	+2°C to +8°C

Note: InsectExpress Sf9-S2 is light sensitive. The medium should be protected from light during shipping and storage.