Cat. No.	Volume
Hygromycin B	P15-014	500 mg
	P21-014	1 g
Hygromycin B Solution (50 mg/ml)	P02-015	20 ml

Hygromycin B is a unique aminoglycoside antibiotic produced by Streptomyces hygroscopicus. It can be used as a selective antibiotic in molecular genetics and in cell culture experiments to inhibit the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) as well as mammalian cells. Hygromycin B inhibits the protein synthesis at the translocation step by interfering with the 70S ribosome causing misreading of the mRNA. It is effective on most bacteria, fungi and higher eukaryotes.

• Selection antibiotic
• Hygromycin B phosphotransferase inactivates Hygromycin B
• Transfections with the hph gene are resistant to Hygromycin B
• Transient and/or stable transfected cells

• Antibiotic with a broad spectrum of activity
• Toxic against all prokaryotes and eukaryotes
• High purity
• High activity
• Packed in dark glass bottles
• Sterile solution

Recommended final concentration: 50 – 800 µg/ml

Purity	> 85.0 %
Potency	> 900 U/mg
Shelf Life	2 years
Storage	+2°C to +8°C

Osmolality	80 – 140 mOsmol/kg
Sterility	tested
Shelf Life	2 years
Storage	+2°C to +8°C

Note:
Hygromycin B is light sensitive. Hygromycin B should be protected from light during shipping and storage.