Cat. No.	Volume
Ham's F-10 Ready Mix	R11-821	100 ml

The karyotyping of chromosomes isolated from lymphocytes, amniocytes and chorionic villi cells is an important tool in cytogenetics. For chromosomal ana­lysis peripheral blood lymphocytes and amniocytes are cultivated in media specifically designed to maximize the yield of intact chromosomes.

Ham's F-10 is one of the most frequently used cul­ture media in cytogenetics. To cultivate amniocytes and chorionic villi cells, important supplements such as foetal bovine serum (FBS) and antibiotics need to be added. However, if you prepare your own media, a considerable amount of time is spent on testing the FBS. Ham's F-10 Ready Mix is a ready to use medium consisting of Ham's F-10 basal medium, FBS (pre-tested on amniocytes and chorionic villi cells), L-glutamine and gentamicin. It is a cost-effective alter­native to other special media used in cytogenetic labo­ratories. This complete medium, when supplemented with phytohaemagglutinin (PHA), is also suitable for the rapid growth of peripheral blood lymphocytes.

The reliability, safety and quality assurance testing of FBS is performed in two independent cytogenetic labo­ratories. Primary amnion cell growth is screened and the performance is compared to a reference standard.

Extensive quality control tests assure constant high product quality. PAA manufactures cell culture pro­ducts according to current GMP. Further quality control and quality assurances are carried out to the highest standards as required by the biopharmaceutical indu­stry.

• Ready to use medium
• Contains pre-tested FBS
• Suitable for growing amniocytes and chorionic villi cells as well as lymphocytes
• Reduced workload and costs
• Optimized reproducibility
• For chromosome analysis
• For karyotyping
• For further analysis including FISH analysis
• Manufactured according to cGMP