Cat. No.	Volume
G5 Supplement
100x Concentrate	F001-003	1 ml

Foetal Bovine Serum contains factors which can inhibit growth of neuronal cells of the nervous system. For cultivation of glial and primary cell lines of astrocyte phenotype a specific serum free supplement has been developed. The G5 Supplement with EGF and FGF  1ml ­optimized for serum free proliferation and differentiation of ­neuronal stem cells into neurons and astrocytes.

G5 Supplement is a multi-purpose supplement for serum free cultivation of glial cells and neuronal stem cells.

Glias are secondary cells involved in nourishment and structural support of neurons. The main cell types of glial cells consist of astrocytes which are responsible for producing the myelin sheaths and oligo­dendrocytes which provide neurons with food. For detailed studies of different types of glial cells a ­defined serum substi­tute must be used. With G5 Supplement a complete set of optimized growth stimu­lating factors is availab­le. It is recommended to combine G5 Supplement with the basal media DMEM or DMEM/Ham’s F-12.

Neuronal stem cells can be grown under serum free conditions with a complex mixture of growth factors and hormones. G5 Supplement can be used for proli­feration of neuronal stem cells. The proliferation rate can be increased by addition of Neuronal Stem Cell Supplement. For differentiation of neuronal stem cells G5 Supplement must be combined with B27 NeuroMix a serum free supplement containing vitamin A which is necessary for neurogenesis. Depending on the types of cells being cultivated G5 Supplement can be substituted with N2 Supplement.

G5 Supplement contains insulin, human transferrin, selenite, hydrocortisone, EGF and FGF.

• Multi-purpose serum substitute
• Optimized hormone composition
• For cultivation of glial cells
• For proliferation of neuronal stem cells
• Important component for differentiation of neuronal stem cells
• Cultivation of glial and primary cell lines of astrocyte phenotype