| Cat. No. | Volume | |
| CryoMaxx S | J05-013 | 50 ml |
| CryoMaxx SF | J05-010 | 50 ml |
General
Cryopreservation is a process used to assure an adequate stock of cells and serves as back up in case of any contaminations of the working cell lines. With a stock of frozen cells constant quality can be guaranteed without change of cellular and genetic features. Only under cyropreserved conditions the cells can maintain homogenous populations because the cellular metabolisms are reduced to a minimum without any genetic drifts.
Classical Cryopreservation Media
Cryopreservation is a procedure to stabilize cells at cryogenic temperatures for long term storage in liquid nitrogen. During the freezing process the usage of cryoprotective additives protects fragile membranes and organelles of the cells by minimizing the damage associated with ice crystal formation.
Classical cryopreservation media contain DMSO with stabilizers such as FBS or methylcellulose which increase the viability after thawing.
CryoMaxx S and SF
Serum containing cryopreservation media are most often used in academic research for cell preservation. CryoMaxx S is a classical serum containing medium with 10% DMSO.
For applications that require serum free conditions freezing media with other cryoprotectants are needed. CyroMaxx SF is a serum free cryopreservation medium that contains 10% DMSO and methylcellulose for cell stabilization.
Composition
CryoMaxx S contains DMEM/Ham’s F12 with 20% FBS and 10% DMSO.
CryoMaxx SF is based on DMEM/Ham’s F12 containing methylcellulose and 10% DMSO.
Features
- Ready to use solution
- Contains cryoprotective agents to minimize dehydration effects
- Serum free and serum containing cryopreservation media
- Simple freezing protocol
- High viability of cells after thawing
