Cat. No. Volume
CoolStar J11-007 100 ml
     
CoolStar Kit J12-078  
CoolStar   100 ml
CoolStar Rinse   100 ml

General

Hypothermic preservation is a procedure to stabilize cells and organs at +2°C to +8°C. The goal of cold storage is to induce hypothermia in order to suppress the rate of cell deterioration. An optimal preservation solution contains components to reduce cell swelling (colloids), buffer to maintain pH balance, antioxidants to scavenge oxygen free radicals and adenosine ­triphosphate precursors to provide energy upon reperfusion. The cold storage of tissues and organs with optimized hypothermic preservation media is a pre­requisite for successful animal organ transplantation experiments.

CoolStar

CoolStar has been formulated to decrease the free radical accumulation in tissues and organs. Free radical accumulation leads to cell death in the case of hypothermic preservation.

CoolStar Rinse has been formulated as a flush solution to rinse culture media and native fluids from mammalian cells, tissues and organs. It should be used in combination with CoolStar.

Composition

CoolStar is a new hypothermic medium consisting of some disaccharides (sucrose, mannitol, and glucose), lactobionate, dextran, buffers and other reducing agents which reduce free radicals. CoolStar Rinse is a solution for rinsing of organs and tissues which consists of different disaccharides and buffers.

Applications

  • Optimized medium for hypothermic preservation
  • Preservation of different mammalian cells, tissues and organs

Features

  • Ready to use solution
  • Simple protocol
  • Reduced viscosity
  • Gentle cold storage
  • Transportation of cells
  • Storage of cells and organs at +2°C to +8°C
  • Improved viability of cells after 2 – 5 days storage at +2°C to +8°C

Storage

Shelf Life 1 year
Storage +2°C to +8°C

Note:
CoolStar is a light sensitive solution. It should be ­protected from direct light during shipping and storage.