| Cat. No. | Volume | |
| Bovine Pituitary Extract | F001-006 | 1 ml |
| F050-006 | 5 x 1 ml |
General
Bovine Pituitary Extract (BPE) is an extract prepared from bovine pituitary glands. It is used for primary or stem cell cultivation in serum free or serum reduced conditions where additional supplementation from crude tissue extracts is still required. The extract is used together with other growth factors, hormones and base media to reduce or eliminate serum in cultivation of non-fibroblast cell types.
BPE is offered as an aqueous solution because lyophilization reduces the biological activity of the powder.
Bovine Pituitary Extract harvested from BSE-free animals in New Zealand
The raw material used for the manufacturing of BPE is derived from bovines of New Zealand origin, where no cases of bovine spongiform encephalopathy (BSE) have been reported.
Applications
- Supplement in serum-free culture medium
- Cultivation of epithelial and endothelial cells
- For primary and stem cell cultures
Features
- Maximum mitogenicity
- Consistent high activity
- Source of growth factors and hormones
- New Zealand origin
- Full documentation of sourcing
- Ready to use, sterile liquid
Working Concentration
Recommended concentration: 35-70 mg/ml, depending on the cell type. An optimized basal medium such as MCDB 154 and MCDB 170 with proper hormone supplementation is required to ensure best results.
Product Specifications
| pH | 6.9 - 7.4 |
| Osmolality | 280 - 340 mOsmol/kg |
| Total Protein | > 14 mg/ml |
| Sterility | tested |
| Source | Bovine pituitary glands |
| Appearance | Brown to reddish liquid |
| Shelf Life | 12 months |
| Storage | ≤ -15°C |