Cat. No.	Volume
Blasticidin	P05-017	50 mg
	P11-017	100 mg

Blasticidin is a peptidyl nucleoside antibiotic isolated from broth cultures of Streptomyces griseochromo­genes. It specifically inhibits the protein synthesis in prokaryotic and eukaryotic cells by inhibiting the for­mation of peptide bonds in the ribosomal machinery.

Blasticidin is used to select transfected cells carrying resistance genes. There are three genes which can be used for transformation experiments in mammalian and plant cells.

• The acetyl transferase gene bls
• The deaminase gene bsr from Bacillus cereus
• The deaminase gene BSD from Aspergillus terreus

Blasticidin resistance is conferred by the blasticidin deaminase which converts blasticidin to a non-toxic deaminohydroxy derivative.  

Blasticidin is useful in experiments conducted with mammalian cells but it is poorly active on E.coli.

Formula: C₁₇H₂₆N₈O₅ x HCl

Molecular Weight: 458.5 g/mol

The typical working concentration of Blasticidin is between 3 and 50 µg/ml for mammalian cell selec­tion.