Cat. No. Volume
Blasticidin P05-017 50 mg
  P11-017 100 mg

General

Blasticidin is a peptidyl nucleoside antibiotic used for selection of cells expressing the bsr gene. The anti­biotic is active against many bacteria, yeast and animal cells by interfering with the peptide bound formation in the ribosomal machinery of prokaryotes and ­eukaryotes. Resistance to blasticidin is conferred by the blasticidin resistence gene bsr which codes for ­blasticidin deaminase.

Applications

  • Selection antibiotic used in transfection
  • Inactivation of blasticidin with acetyl transferase and deaminases
  • Cells transfected with plasmids carrying the pac, bls, bsr and bsd genes are resistant against ¬blasticidin

Features

  • Nucleoside antibiotic
  • Inhibition of peptidyl transfer
  • Spectrum: bacteria, various animal cells and yeast
  • High purity
  • High activity

Working Concentration

Recommended final concentration: 3 – 50 µg/ml

Product Specifications

Purity > 98.0 %
Shelf Life 3 years
Storage +2°C to +8°C